产品货号：
Z33735
中文名称：
RPA2抗体
英文名称：
Anti-RPA2 Antibody (Clone#3B2E9)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-RPA2 Antibody (Clone#3B2E9) |
| 指标别名 | REPA2;Replication factor A protein 2;replication protein A2;32kDa;RF A protein 2;RP A p32;RP A p34;RPA2;RPA32;RPA34 |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | RPA2 |
| 克隆号 | 3B2E9 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2a |
| 免疫原 | E.coli-derived human RPA32/RPA2 recombinant protein (Position:Q34-H254). |
| 实际分子量 | 29KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex,which binds to single-stranded DNA (ssDNA),forming a nucleoprotein complex that plays an important role in DNA metabolism,being involved in DNA replication,repair,recombination,telomere maintenance,and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase.The RPA complex protects single-stranded DNA from nucleases,prevents formation of secondary structures that would interfere with repair,and co-ordinates the recruitment and departure of different genome maintenance factors.The heterotrimeric complex has two different modes of ssDNA binding,a low-affinity and high-affinity mode,determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized,and differing in the length of DNA bound.This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus,which interacts with many genome maintenance protein.Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. |
| Uniprot ID | P15927 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 基因名全称 | replication protein A2 |
| 蛋白名全称 | Replication protein A 32kDa subunit |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
相关搜索：RPA2抗体，RPA2单抗，Anti-RPA2 Antibody (Clone#3B2E9)